|
KMID : 0365819740140020147
|
|
Journal of Pusan Medical College
1974 Volume.14 No. 2 p.147 ~ p.159
|
|
|
Histogenesis and Histochemistry of the Mucosubstances in the Vertebrae of the Developin Chicken
|
|
|
|
|
Abstract
|
|
|
In order to study of the histogenesis of the chicken vertebrae and the histochemistry of mucosubstance in the matrices of them, the cervical and thoracic vertebrae were observed in the chick embryos at 4, 7. 10, 13, 16,19th incubation day and those in the chickens at 1, 4, 7, 10th day after hatching.
The mucosubstances were stained with alcian blue (pH 2.5-r1.0), PAS, alcian blue (pH 2.5-.-1.0)-PAS and aldehyde fuchsin (pH 1.7)-alcian blue (pH 2.5).
The results obtained were as follows:
1. The first primordium of the vertebrae appeared already in the 4th day embryos, and it became the precartilages at the 7th day, and further developed to true cartilages in the 7 to 10th day embryos. The cartilage matrices calcified at 16 to 19th day embryos and followed by destruction of them and deposition of new bone, which began from 16 to 19th day embryos and developed gradua llythereafter with the time after hatching.
2. The mucosubstances which identified as sulfated mucin were more concentrated in the matrices of the true cartilage than those in the precartilage.
3. In the calcified regions, the acid mucosubstances were more abundant than in the matrices of the precartilage and true cartilage. Especially the zone of cell hypertrophy contained most abundant acid mucosubstances than any other zone.
4. The neutral mucosubstance can be negligible in the precartilage and true cartilage but it became increased slightly in the early stage of the calcification and also in the zone of endochondral bone deposition.
5. The mucosubstances in the zone of the new bone deposition were composed of mainly neutral mucosubstance and trace of acidic one.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|